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Factor Xa Protease

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Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.

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P8010S 1 mg/ml 50 µg $88.00
P8010L 1 mg/ml 250 µg $353.00
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  • Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate (1,2,3). The most common secondary site, among those that have been sequenced, is Gly-Arg. There seems to be a correlation between proteins that are unstable in E.coli and those that are cleaved by Factor Xa at secondary sites; this may indicate that these proteins are in a partially unfolded state (Walker, I., Riggs, P., unpublished observations). Factor Xa will not cleave a site followed by proline or arginine.

    Product Source

    Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell's viper venom.
    This product is related to the following categories:
    Proteases Products
    This product can be used in the following applications:
    Fusion Protein Cleavage,
    Target Protein Insolubility ,
    Protein Purification,
    Protein Digestion,
    Protein Expression

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      1 μg of Factor Xa will cleave 50 μg of MBP fusion protein test substrate, MBP-ΔSal to 95% completion in a total reaction volume of 50 μl in 6 hours or less at 23°C in 20 mM Tris-HCl (pH 8.0 @ 25°C) with 100 mM NaCl and 2 mM CaCl2.

      Usage Concentration

      1 mg/ml

      Removal

      Factor Xa will bind specifically to benzamidine-agarose.

      Molecular Weight

      Theoretical: 43 kDa

      Deactivation

      Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700) will irreversibly inactivate Factor Xa by covalent attachment at the active site. In a reaction containing 20 μg/ml Factor Xa, 2 μM dansyl-glu-gly-arg-chloromethyl ketone will inactivate >95% of the Factor Xa in 1 minute at room temperature.

    • Product Notes

      1. The test substrate MBP-ΔSal is maltose-binding protein fused to a truncated form of paramyosin, with the amino acids Ile-Glu-Gly-Arg at the fusion joint. Greater than 95% of the fusion protein is cleaved in 6 hours or less.

    • References

      1. Nagai, K. et al. (1985). Proc. Natl. Acad. Sci. USA. 82,
      2. Quinlan, R.A. et al (1989). Cell Sci. 82, 7252-7255.
      3. Eaton, D. et al (1986). Biochemistry. 25, 505-512.

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