HomeFAQsWhy can only 50% of AciI cut and ligated fragments be recleaved by AciI?
FAQ: Why can only 50% of AciI cut and ligated fragments be recleaved by AciI?
AciI has a non-palindromic recognition site. As a result, when DNA is cut with AciI and then ligated, only 50% of these ligated sites regenerate an AciI site.
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