HomeFAQsI've digested my DNA and now want to blunt directly without purifying, can I add the blunting reagents directly to the restriction digest?
FAQ: I've digested my DNA and now want to blunt directly without purifying, can I add the blunting reagents directly to the restriction digest?
We recommend to heat inactivate the restriction enzyme after a restriction digest and prior to the blunting reaction. If the restriction digest has been carried out with enzymes that can be inactivated by a heat treatment, then after the heat inactivation step, add 0.1 volume of dNTPs and 1 ul of Blunting Enzyme and incubate at room temperature for 15 minutes. If the restriction enzymes used are not inactivated by a heat treatment, then the reaction will need to be purified using a commercial purification kit (we recommend the Monarch PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.