- Combine 10-20 µg of glycoprotein, 1 µL of 10X DTT and H2O (if necessary to make a 10 µL total reaction volume.
- Denature glycoprotein by heating reaction at 95°C for 3 minutes.
- Make a total reaction volume of 20 µL by adding 2 µL 10X GlycoBuffer 2, H2O and 1 µL Endo D.
- Incubate reaction at 37°C for 1 hour.