HomeFAQsWhat are the optimal incubation temperature and time for lysing cells with NEBExpress® T4 Lysozyme?
FAQ: What are the optimal incubation temperature and time for lysing cells with NEBExpress® T4 Lysozyme?
Cells should be lysed with T4 Lysozyme at room temperature or 4˚C for 5-10 minutes with gentle shaking. Incubation at 37˚C is not recommended to avoid potential induction of protease activity.
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