HomeFAQsWhat size DNA can be purified with the Monarch® Spin Columns S1A (NEB #T2037)?
FAQ: What size DNA can be purified with the Monarch® Spin Columns S1A (NEB #T2037)?
Recovery of DNA from 50 bp to 25 kb can be accomplished with the Monarch Spin Columns S1A, supplied in both the Monarch Spin DNA Gel Extraction Kit (NEB #T1120) and the Monarch Spin PCR & DNA Cleanup Kit (5 µg) (NEB #T1130). Efficiency decreases with longer DNA due to tighter binding to the matrix. The recovery of DNA ≥ 15 kb can be improved by modifying the elution method, using heated elution buffer (50°C) and extending incubation times. For optimal recovery of large DNA fragments, please follow the instructions in the manual.
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