Home FAQs What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007, #M3019, #M3029 and #L4001)?

FAQ: What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007, #M3019, #M3029 and #L4001)?

These Luna products are all optimized for one-step RT-qPCR by probe detection. NEB #M3019/#M0329 are one-tube 4X master mixes The NEB #E3006/#E3007 products contain separate tubes for the 2X reaction mix and 20X enzyme mix. Also, the M3019 product includes not only dUTP, but also a thermolabile UDG, to fully enable carryover prevention. NEB #L4001 is a lyophilized version of NEB#M3019 and is available in vials or 96-well plates. NEB #E3007 and #M3029 lack a universal ROX passive reference dye. Available mixes differ with regards to format, storage temperature, and inclusion of thermolabile UDG for carryover prevention, as outlined in the table below.
 
  NEB #M3052
Luna® Direct Probe One-Step RT-qPCR 4X Mix with UDG 		  NEB #M3053
Luna® Direct Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)  		 NEB #M3019
Luna Probe One-Step RT-qPCR 4X Mix with UDG
 NEB #M3029
Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)		 NEB #E3006
Luna Universal Probe One-Step RT-qPCR Kit		 NEB #E3007
Luna Probe One-Step RT-qPCR Kit (No ROX) 		NEB #L4001
LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG
Format 4X Master Mix  4X Master Mix  4X Master Mix
 4X Master Mix
 2X Reaction Mix
20X Enzyme Mix		 2X Reaction Mix
20X Enzyme Mix		 Lyophilized Master Mix; rehydrate at 2-4X
Carryover prevention included Y
(dUTP and thermolabile UDG included)

Y
(dUTP and thermolabile UDG included)

 Y
(dUTP and thermolabile UDG included)
 Y
{dUTP and thermolabile UDG included)		 N
(dUTP included; requires UDG addition)		 N
(dUTP included; requires UDG addition)		 Y
(dUTP and thermolabile UDG included)
Normalization dye
(universal ROX)		 Y  N  Y N Y N Y
Multiplexing Up to 6 plex  Up to 6 plex  Y (up to 5-plex)
 Y (up to 5-plex)
 Y (up to 5-plex) Y (up to 5-plex) Y (up to 5-plex)
Maximum sample volume
(20 µl RT-qPCR reaction)		 up to 10 µl  up to 10 µl  up to 10 µl
 up to 10 µl
 up to 8 µl up to 8 µl up to 10 µl
Standard cycling condition 25°C for 30 sec. (UDG treatment);
55°C for 5 min;
95°C for 1 min;
45 cycles of 95°C for 5 sec., 60°C for 10-30 sec. 		 25°C for 30 sec. (UDG treatment);
55°C for 5 min;
95°C for 1 min;
45 cycles of 95°C for 5 sec., 60°C for 10-30 sec. 		 25°C for 30 sec. (UDG treatment);
55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.
 25°C for 30 sec. (UDG treatment);
55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.
 55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.		 55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.		 25°C for 30 sec. (UDG treatment);
55°C for 10 min;
95°C for 1 min;
45 cycles of 95°C for 10 sec., 60°C for 30 sec.
Storage temperature -20°C  -20°C  -20°C
 -20°C
 -20°C
 -20°C
 Room temperature (15°C to 25°C)