Protocol for WarmStart Afu Uracil-DNA Glycosylase (UDG) (NEB #M1282)
- Set-up the reaction as follows:
dU-containing DNA up to 1800 pmol Thermopol® II Reaction Buffer (10X) 5 µl WarmStart® Afu Uracil-DNA Glycosylase (UDG) 1 µl (2 units) H2O up to 50 µl - Incubate at 65°C for 30 minutes.