Bst DNA Polymerase, Large Fragment
Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
This product is available in a glycerol-free format. Contact us for more information.
The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)
- Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
- Suitable for applications requiring thermophilic strand displacement
- Supplied with ThermoPol® Reaction Buffer for high product yields in demanding conditions
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Product Information
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the
5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.Highlights
- Isolated from a recombinant source
- Sequencing through problematic secondary structures
- Supplied with 10X Reaction Buffer
Product Source
Bst Polymerase, Large Fragment is prepared from an E. coli strain containing a genetic fusion of the Bacillus stearothermophilus DNA Polymerase gene, lacking the 5´ → 3´ exonuclease domain, and the gene coding for E. coli maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion of the fusion is cleaved off in vitro. The remaining polymerase is purified free of MBP (1).- This product is related to the following categories:
- Isothermal Amplification & Strand Displacement Products
- This product can be used in the following applications:
- Whole Genome Amplification,
- Loop-Mediated Isothermal Amplification,
- Isothermal Amplification
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Product Citation Tool
Additional Citations
- Trisadee Khamlor, Petai Pongpiachan, Rangsun Parnpai, Kanchana Punyawai, Siwat Sangsritavong, Nipa Chokesajjawatee (2015) Bovine embryo sex determination by multiplex loop-mediated isothermal amplification. Theriogenology; 83, 891-6. PubMedID: 25542460, DOI: 10.1016/j.theriogenology.2014.11.025
- DoKyung Lee, Eun Jin Kim, Paul E Kilgore, Soon Ae Kim, Hideyuki Takahashi, Makoto Ohnishi, Dang Duc Anh, Bai Qing Dong, Jung Soo Kim, Jun Tomono, Shigehiko Miyamoto, Tsugunori Notomi, Dong Wook Kim, Mitsuko Seki (2015) Clinical Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Neisseria meningitidis in Cerebrospinal Fluid. PLoS One; 10, e0122922. PubMedID: 25853422, DOI: 10.1371/journal.pone.0122922
- Manabu Nemoto, Yoshinori Morita, Hidekazu Niwa, Hiroshi Bannai, Koji Tsujimura, Takashi Yamanaka, Takashi Kondo (2015) Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification. J Virol Methods; 215-216, 13-6. PubMedID: 25682750, DOI: 10.1016/j.jviromet.2015.02.001
- Mohammad Reza Allahyar Torkaman, Kazunari Kamachi, Vajihe Sadat Nikbin, Masoumeh Nakhost Lotfi, Fereshteh Shahcheraghi (2015) Comparison of loop-mediated isothermal amplification and real-time PCR for detecting Bordetella pertussis. J Med Microbiol; 64, 463-5. PubMedID: 25596118, DOI: 10.1099/jmm.0.000021
- Aongart Mahittikorn, Hirotake Mori, Supaluk Popruk, Amonrattana Roobthaisong, Chantira Sutthikornchai, Khuanchai Koompapong, Sukhontha Siri, Yaowalark Sukthana, Duangporn Nacapunchai (2015) Development of a Rapid, Simple Method for Detecting Naegleria fowleri Visually in Water Samples by Loop-Mediated Isothermal Amplification (LAMP). PLoS One; 10, e0120997. PubMedID: 25822175, DOI: 10.1371/journal.pone.0120997
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