Taq DNA Polymerase with ThermoPol® Buffer
Taq DNA Polymerase is the industry standard for PCR
- Ideal for routine PCR applications
- ThermoPol Buffer delivers high product yield under demanding conditions
- Exceptional value in terms of cost per unit
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Product Information
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Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.Highlights
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1- This product is related to the following categories:
- Taq DNA Polymerase
- This product can be used in the following applications:
- Colony PCR,
- A-tailing,
- Polymerases for DNA Manipulation,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR, PCR
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Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration -
NEB # Stored at (°C) M0267S -20 Component Name Component # Stored at (°C) Amount Concentration Taq DNA Polymerase with ThermoPol® Buffer M0267SVIAL -20 1 x 0.08 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 2 x 1.5 ml 10 X
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NEB # Stored at (°C) M0267L -20 Component Name Component # Stored at (°C) Amount Concentration Taq DNA Polymerase with ThermoPol® Buffer M0267LVIAL -20 1 x 0.4 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 4 x 1.5 ml 10 X
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NEB # Stored at (°C) M0267X -20 Component Name Component # Stored at (°C) Amount Concentration Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 1 x 0.8 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 8 x 1.5 ml 10 X
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NEB # Stored at (°C) M0267E -20 Component Name Component # Stored at (°C) Amount Concentration Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 5 x 0.8 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 40 x 1.5 ml 10 X
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Reaction Conditions
1X ThermoPol® Reaction Buffer
1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°CHeat Inactivation
NoMolecular Weight
Theoretical: 94000 daltons
5' - 3' Exonuclease
Yes3' - 5' Exonuclease
NoStrand Displacement
+Resulting Ends
- Single-base 3´ Overhangs
Unit Assay Conditions
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.Error Rate
~ 285x10-6bases -
Advantages and Features
Application Features
- PCR
- Primer extension
- Colony PCR
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Related Products
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Product Notes
- 5'→3' flap endonuclease activity destroys displaced strand.
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References
- Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
- Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
- Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
- Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
- Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
- Saiki R.K. et al. (1985). Science. 230, 1350-1354.
- Powell, L.M. et al. (1987). Cell. 50, 831-840.
- Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
- Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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FAQs
- What ends will my PCR products have?
- How should I determine the appropriate annealing temperature for my reaction?
- What should the final primer concentration be in my PCR?
- What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
- What are the stability and storage requirements for NEB's Taq and OneTaq DNA Polymerases?
- Is this Taq DNA Polymerase product compatible with other NEB Buffers?
- Can Taq/OneTaq® DNA Polymerases be used for nick translation?
- How can I optimize my PCR when using Taq DNA Polymerase?
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Troubleshooting
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Tech Tips
Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
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Citations & Technical Literature
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Citations
Product Citation Tool
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Quality, Safety & Legal
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] -
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M0267S_L_X_E_v1_0141506
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Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Trademarks
NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
IGEPAL® is a registered trademark of Rhodia Operations.
TRITON® is a registered trademark of Union Carbide Corporation
TWEEN® is a registered trademark of Uniqema Americas, LLC.
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