SphI-HF®
neb31 cloned at NEB recombinant engineered time saver dil_B 37 65 Heat

SphI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10141297. Learn more.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


NEB high fidelity restriction endonuclease that recognizes the sequence GCATG^C

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance
  • 100% activity in rCutSmart Buffer
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • Reduced star activity
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: GCATG/C
Special Offer for Q4 Ladders
Catalog # Concentration Size List Price Your Price Quantity
R3182S 20,000 units/ml 500 units $75.00
R3182L 20,000 units/ml 2,500 units $308.00
R3182M 100,000 units/ml 2,500 units $308.00
Please enter a quantity for at least one size
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