Monarch^® Mag Viral DNA/RNA Extraction Kit

The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and respiratory swab samples. The kit combines the efficiency of silica-based nucleic acid purification with the ease of use of magnetic beads. Manual and automated workflows allow samples to be processed in microfuge tubes or 96-well plates. Kit sizes align to 96-well formats (100 preps, 600 preps, and 1800 preps), and the protocol is compatible with high throughput automation on a variety of platforms, including the KingFisher Flex magnetic particle processor and Agilent Bravo and MGISP liquid handler platforms.

Need automation support or a script? Submit a request using the Monarch Automation Support & Script Request form. Automation scripts are currently available for select platforms. 

* Viral DNA and RNA are purified in parallel. Preparation of DNA-free RNA or RNA-free DNA requires further treatment with the appropriate nuclease (not supplied).
** The sample input volume may be scalable to accommodate larger sample volumes. Further workflow optimization may be required.
*** Use of carrier RNA is recommended for recovery of low amounts of viral nucleic acid. Carrier RNA should be omitted if the downstream application utilizes poly(A) RNA enrichment; however, viral nucleic acid recovery may be reduced.

Figure 1: Monarch Mag Viral DNA/RNA Extraction Kit Workflow

 
Silica-coated Monarch Mag Beads M1 are utilized for highly sensitive nucleic acid capture from samples containing few target molecules. Silanol groups on the bead shell provide an optimal binding surface for nucleic acids, and the uniform, submicron bead size offers a high surface area with abundant binding sites. Beads are supplied as a monodispersed suspension. The superparamagnetic properties of the bead core result in a fast magnetic response, contributing to ease of handling during use and compatibility with automation

An optimized viral nucleic acid extraction procedure employs a sample lysis step followed by a simple bind-wash-elute process. Samples are treated with Proteinase K and then mixed with a lysis buffer/bead mixture containing lysis buffer, carrier RNA, isopropanol, and magnetic beads, which promote binding of viral nucleic acid onto the silica-coated beads. Carrier RNA is utilized as a co-precipitant in the workflow to enhance the recovery of low amounts of viral nucleic acid. After binding of viral DNA and RNA to the magnetic beads, the beads are washed to remove contaminants, and nucleic acid is eluted in nuclease-free water. Purified viral nucleic acid is suitable for downstream applications, including qPCR/RT-qPCR, ddPCR, and library prep for Next Generation Sequencing (NGS).

Figure 2: Complete SARS-CoV-2 genome coverage from wastewater samples using the Monarch Mag Viral DNA/RNA Extraction Kit

Figure 3: Performance comparison of Monarch Mag Viral DNA/RNA Extraction Kit with other suppliers demonstrates high reproducibility and sensitivity of the Monarch kit

Figure 4: qPCR on DNA extracted using the Monarch Mag Viral DNA/RNA Extraction Kit demonstrates accurate detection and quantification of target DNA viruses

Figure 5: RT-qPCR on RNA extracted using the Monarch® Mag Viral DNA/RNA Extraction Kit demonstrates detection of multiple viruses from the same sample

This product is related to the following categories:

Magnetic Bead-Based Nucleic Acid Purification,

Nucleic Acid Purification

This product can be used in the following applications:

Nucleic Acid Purification